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Title   말징버섯 배양균사체의 항암성분 (Antitumor Components of the Cultured Mycelia of Calvatia craniformis )
Publicationinfo   1992 Jan; 024(01): 1-19.
Key_word   Caleatia craniformis, B-form polysacchaide, antitumor activity, immunopotentia- tion, superoxide anion, hemolytic plaque assay, calvatan
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Abstract   To find antitumor components from higher fungi, the cultured mycelia of Calvatia craniformis were extracted with hot water. The water soluble fraction was purified and separated by DEAE-cellulose ion exchange chromatography, Sepharose CL-4B gel filtration method and Con A Sepharose 4B affinity chromatagraphy. The separated fractions were designated A, B, C, D, E and F. The tumor inhibition ratio of these six fractions was 41.7-74.1% at the dose of 10 mg/kg/day or 20 mg/kg/day for 10 days. Fr. F(B-form) showed highest antitumor activity among the six fractions. When Fr. D was examined for immunopotentiation activity, it in- creased the amount of the superoxide anion from activated macrophages to 1.4 fold and the number of plaques in hemolytic plque assay to 3.1 fold, respectively. These results indicate that the antitumor activity was exerted through immunopotentiation. Its chemical analysis showed 87.2% polysaccharide, 1.9% protein and 1.3% hexosamine. The polysaccharide consisted of glucose, galactose, mannose and xylose. Molecular weight of Fr. D was found to be 55,000 dalton. This was named calvatan.
저 자   김병각(Byong Kak Kim),권지연(Ji Yun Kwun),박영인(Young In Park),복진우(Jin Woo Bok),최응칠(Eung Chil Choi)